Journal: PLoS ONE

Article Title: Conserved Expression of the Glutamate NMDA Receptor 1 Subunit Splice Variants during the Development of the Siberian Hamster Suprachiasmatic Nucleus

doi: 10.1371/journal.pone.0037496

Figure Lengend Snippet: Insert components and commonly used nomenclature for the eight NMDAR1 splice variants.

Article Snippet: Immunohistochemical analysis (IHC) of NR1-immunoreactivity was performed as previously described using an anti-NR1 rabbit polyclonal antibody (Chemicon, Temecula, CA, USA) at a dilution of 1∶100.

Techniques:

Journal: PLoS ONE

Article Title: Upregulation of Protein Phosphatase 2A and NR3A-Pleiotropic Effect of Simvastatin on Ischemic Stroke Rats

doi: 10.1371/journal.pone.0051552

Figure Lengend Snippet: representative blots showing the levels of phosphrylation of NR1 (ser896, ser897) and total NR1 in each group. B: semiquantitative analysis of the levels of phosphrylation of NR1 (ser896, ser897) and total NR1; values expressed as means ± SEM of 5 rats; ** P <0.01, MCAO vs . sham group, vehicle+MCAO vs . sham group; ## P <0.01, sim+MCAO vs . MCAO group; # P <0.05, sim+MCAO vs . MCAO group; && P <0.01, sim+MCAO vs . vehicle+MCAO group; & P <0.05, sim+MCAO vs . vehicle+MCAO group. Sim, Simvastatin; pNR1ser896, phosphor NR1 (ser896); pNR1ser897, phosphor NR1 (ser897).

Article Snippet: The primary antibodies were as follows: mouse anti-PP2A, C subunit monoclonal antibody (1∶2000; Millipore, MA, USA), rabbit anti-NR1 monoclonal antibody (1∶1000; Cell Signaling Technology, MA, USA), rabbit anti-phospho NR1 (Ser896) polyclonal antibody (1∶1000; Millipore, MA, USA), rabbit anti-phospho NR1 (Ser897) polyclonal antibody (1∶1000; Millipore, MA, USA), rabbit anti-NR3A polyclonal antibody (1∶1000; Millipore, MA, USA), and mouse anti-β-actin polyclonal antibody (1∶3000; Beyotime Institute of Biotechnology, Haimen, China).

Techniques:

Journal: PLoS ONE

Article Title: Upregulation of Protein Phosphatase 2A and NR3A-Pleiotropic Effect of Simvastatin on Ischemic Stroke Rats

doi: 10.1371/journal.pone.0051552

Figure Lengend Snippet: representative blots showing the levels of phosphrylation of NR1 (ser896, ser897) and total NR1 in each group. B: semiquantitative analysis of the levels of phosphrylation of NR1 (ser896, ser897) and total NR1; values expressed as means ± SEM of 5 rats; ** P <0.01, MCAO vs . sham group, vehicle+MCAO vs . sham group; ## P <0.01, sim+MCAO vs . MCAO group; # P <0.05, sim+MCAO vs . MCAO group; && P <0.01, sim+MCAO vs . vehicle+MCAO group; & P <0.05, sim+MCAO vs . vehicle+MCAO group. Sim, Simvastatin; pNR1ser896, phosphor NR1 (ser896); pNR1ser897, phosphor NR1 (ser897).

Article Snippet: The primary antibodies were as follows: mouse anti-PP2A, C subunit monoclonal antibody (1∶2000; Millipore, MA, USA), rabbit anti-NR1 monoclonal antibody (1∶1000; Cell Signaling Technology, MA, USA), rabbit anti-phospho NR1 (Ser896) polyclonal antibody (1∶1000; Millipore, MA, USA), rabbit anti-phospho NR1 (Ser897) polyclonal antibody (1∶1000; Millipore, MA, USA), rabbit anti-NR3A polyclonal antibody (1∶1000; Millipore, MA, USA), and mouse anti-β-actin polyclonal antibody (1∶3000; Beyotime Institute of Biotechnology, Haimen, China).

Techniques: